Having found in my home a box of old… see very old (between 8 and 13 years old) equipment for growing mushrooms, I will try to get a few things out of it.
I will try to do a liquid culture to see if it will come back to life, I start with the rest of a commercial syringe of PV (P. Cubensis "Pura Vida")
(I am not going to recall here all the hygiene and environmental measures that this requires... that is not the goal and in this topic and I only respected a minimum, cleaning of hands, utensil and space working with alcohol, burning a needle with a candle, I had nothing else…)
Start of work on 21/05 around 2 p.m.
- 200ml H20
- 8.5gr Honey
- Mix well- 15min at 15 psi under pressure (+- 30 min of cooking in all)
- Allow to cool before opening the pressure cooker- Allow the liquid to cool if it is still a little hot…
Note: there are small deposits floating, of honey I presume… caramelized? at the time I preferred acacia honey, but I did not have any on hand this time.
- Injection of 2ml of 10 volume hydrogen peroxide
- Mix well- Injection of spores
Note: I hope it had cooled down enough… impatient that I am.
- Storage in the dark at 20/25°
A few hours later, I had fun making 2 spore syringes with old prints (one from me and one from a friend) from 2008 and I still have a commercial syringe background from "Ecuador". All this in order to do other tests when I can. I'm curious to see if anything survived.
this was all done as an amateur...
I was better equipped at that time...
We'll see if that motivates me to re-equip myself
to be continued (if this topic has something to do here... i'm not even sure)
I will try to do a liquid culture to see if it will come back to life, I start with the rest of a commercial syringe of PV (P. Cubensis "Pura Vida")
(I am not going to recall here all the hygiene and environmental measures that this requires... that is not the goal and in this topic and I only respected a minimum, cleaning of hands, utensil and space working with alcohol, burning a needle with a candle, I had nothing else…)
Start of work on 21/05 around 2 p.m.
- 200ml H20
- 8.5gr Honey
- Mix well- 15min at 15 psi under pressure (+- 30 min of cooking in all)
- Allow to cool before opening the pressure cooker- Allow the liquid to cool if it is still a little hot…
Note: there are small deposits floating, of honey I presume… caramelized? at the time I preferred acacia honey, but I did not have any on hand this time.
- Injection of 2ml of 10 volume hydrogen peroxide
- Mix well- Injection of spores
Note: I hope it had cooled down enough… impatient that I am.
- Storage in the dark at 20/25°
A few hours later, I had fun making 2 spore syringes with old prints (one from me and one from a friend) from 2008 and I still have a commercial syringe background from "Ecuador". All this in order to do other tests when I can. I'm curious to see if anything survived.
this was all done as an amateur...
I was better equipped at that time...
We'll see if that motivates me to re-equip myself
to be continued (if this topic has something to do here... i'm not even sure)